Technical Data
V2110-16
VEGF Receptor 1 (Flt1, VEGFR1) Soluble (Vascular Endothelial Growth Factor Receptor 1, sVEGFR-1, sFlt1)
Description:
Angiogenesis, the formation of new blood vessels, is regulated by the balance between positive and negative endothelial regulatory factors. Vascular endothelial factor receptor-1 (VEGFR-1), also known as FMS-like tyrosine kinase 1 (Flt-1), is a transmembrane receptor tyrosine kinase with high affinity for vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF) (1-3). Binding of VEGF to VEGFR-1 produces receptor dimerization and subsequent signal transduction. Full length VEGFR-1 is characterized by the presence of seven extracellular immunoglobulin-like domains containing the ligand binding region, a single short transmembrane sequence, and an intracellular region with a tyrosine kinase domain (2). VEGF receptors are expressed by vascular endothelial cells and peripheral blood monocytes (4), and have also been observed in colon carcinoma cell lines and colorectal cancer liver metastases (5). In addition to the full-length form of the receptor, a soluble form (sVEGFR-1 or sFlt-1) is generated by alternative splicing of Flt-1 mRNA. This naturally occurring, soluble form of VEGFR-1 is truncated on the C-terminal side of the sixth extracellular Ig-like domain (6), and has been described in various locations, including the serum and plasma of normal males and females, the serum of pregnant women, placenta, and breast tumor tissues (7,8). Soluble VEGFR-1 is an antagonist of VEGF action and an inhibitor of the VEGF signaling pathway, which has been characterized as one of the most important endothelial regulators in human tumor angiogenesis.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1ug/ml
Western Blot: 1-5ug/ml. Band observed at ~130kD as well as a few bands at lower molecular weight.
Optimal dilutions to be determined by the researcher.
Positive Control: SW480 cell lysates.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.25mg/ml
Immunogen:
Synthetic peptide derived from the C-terminal region of the soluble form of the human VEGF Receptor-1 (sVEGFR-1, sFlt-1) protein.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes the soluble form of human VEGF Receptor-1. Reactivity has been con rmed with human SW480 colorectal adenocarcinoma cell lysates.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Devries C, et al. Science 255:989-991, 1992. 2. Thomas KA. J Biol Chem 271(2):603-606, 1996. 3. Neufeld G, et al. FASEB J 13:9-22, 1999. 4. Sawano A, et al. Blood 97(3):785-791, 2001. 5. Warren RS, et al. J Clin Invest 95(4):1789-1797, 1995. 6. Kendall R, Thomas KA. PNAS 90:10705-10709, 1993. 7. Barleon B, et al. Angiogenesis 4(2):143-154, 2001. 8. Toi M, et al. Int J Cancer 98(1):14-18, 2002. 9. Hornig C, et al. Lab Invest 80(4):443-454, 2000.