Technical Data
V2110-16E
VEGF Receptor 1 (Flt1, VEGFR1) Soluble (Vascular Endothelial Growth Factor Receptor 1, sVEGFR-1, sFlt1)
Description:
VEGFR-1 (vascular endothelial growth factor receptor 1) is a 180-kDa membrane receptor tyrosine kinase that is the receptor for vascular endothelial growth factor (VEGF), VEGFB and PGF. VEGF is a key regulator of physiological angiogenesis during embryogenesis, skeletal growth and reproductive functions. VEGF has also been implicated in pathological angiogenesis associated with tumors, intraocular neovascular disorders and other conditions. VEGFR-1 has seven extracellular Ig-like domains; the second domain from the N-terminus is necessary and sufficient for high affinity VEGF binding. The biological effects of VEGF are mediated by VEGFR-1 and VEGFR-2, and two major tyrosine phosphorylation sites at Tyr 1213 and Tyr 1242 and two minor tyrosine phosphorylation sites at Tyr 1327 and Tyr 1333 exist in VEGFR-1.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, ELISA and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500-1:1000
Immunohistochemistry (Paraffin): 1: 250
Immunocytochemistry: 1: 250
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG6k398Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to residues in the N-terminus of human VEGFR1.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Specificity:
Recognizes human VEGFR1 at ~180kD. Detects both VEGFR1 and its splice isoform sFlt1. Species Crossreactivity: mouse and rat (tested in Western Blot only)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ferrara, N. et al. The biology of VEGF and its receptors. Nat. Med. 9: 669–676 (2003). 2. Starovasnik, M.A., et al. Solution structure of the VEGF-binding domain of Flt-1: comparison of its free and bound states. J Mol Biol. 293: 531–44 (1999). 3. Ito, N., et al. Identification of vascular endothelial growth factor receptor-1 tyrosine phosphorylation sites and binding of SH2 domain-containing molecules. J Biol Chem. 273: 23410–8 (1998).