Technical Data
VEGF Receptor 2 (CD309 antigen, Fetal liver kinase 1, FLK1, KDR, Kinase insert domain receptor, KRD1, Ly73, Protein tyrosine kinase receptor FLK1, Vascular endothelial growth factor receptor 2, VEGFR, VEGFR2)
Vascular endothelial growth factor receptor 2 (VEGFR-2, KDR, Flk-1) is a major receptor transducing the effects of VEGF into endothelial cells. Upon binding to specific ligands, VEGFR-2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of the adaptor proteins, including Shc, GRB2, PI3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The signaling of VEGFR-2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and prouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (4).

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight.

Immunoprecipitation: 1:50

Optimal dilutions to be determined by the researcher.

Molecular Weight:

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Not determined
Synthetic phospho-peptide (KLH coupled) corresponding to residues surrounding Tyr951 of human VEGF receptor 2.
Purified by Protein A and peptide affinity chromatography.
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Detects overexpressed human VEGFR-2 only when phosphorylated at tyrosine 951. Does not crossreact with other phosphorylated receptor tyrosine kinases. Species Crossreactivity: Predicted to crossreact with mouse based on 100% sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Meyer, M., et al., EMBO J. 18: 363-374 (1999). 2. Dougher-Vermazen, M., et al., Biochem. Biophys. Res. Commun. 205: 728-738 (1994). 3. Kroll, J. and Waltenberger, J., J. Biol. Chem. 272: 32,521-32,527 (1997). 4. Karkkainen, M.J. and Petrova, T., Oncogene 19: 5,598-5,605 (2000). 5. Rahimi, N., et al., (2000) J. Biol. Chem. 275: 16,986-16,992.