Technical Data
V2110-18A
VEGF Receptor 2 (CD309 antigen, Fetal liver kinase 1, FLK1, KDR, Kinase insert domain receptor, KRD1, Ly73, Protein tyrosine kinase receptor FLK1, Vascular endothelial growth factor receptor 2, VEGFR, VEGFR2)
Description:
Vascular endothelial growth factor receptor 2 (VEGFR-2, KDR, Flk-1) is a major receptor transducing the effects of VEGF into endothelial cells. Upon binding to specific ligands, VEGFR-2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of the adaptor proteins, including Shc, GRB2, PI3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The signaling of VEGFR-2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and prouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (4).

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight.

Immunoprecipitation: 1:50

Optimal dilutions to be determined by the researcher.

Molecular Weight:
180kD

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic phospho-peptide (KLH coupled) corresponding to residues surrounding Tyr951 of human VEGF receptor 2.
Purity:
Purified by Protein A and peptide affinity chromatography.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity:
Detects overexpressed human VEGFR-2 only when phosphorylated at tyrosine 951. Does not crossreact with other phosphorylated receptor tyrosine kinases. Species Crossreactivity: Predicted to crossreact with mouse based on 100% sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Meyer, M., et al., EMBO J. 18: 363-374 (1999). 2. Dougher-Vermazen, M., et al., Biochem. Biophys. Res. Commun. 205: 728-738 (1994). 3. Kroll, J. and Waltenberger, J., J. Biol. Chem. 272: 32,521-32,527 (1997). 4. Karkkainen, M.J. and Petrova, T., Oncogene 19: 5,598-5,605 (2000). 5. Rahimi, N., et al., (2000) J. Biol. Chem. 275: 16,986-16,992.