Technical Data
V2110-18F
VEGFR-2 (Vascular Endothelial Growth Factor Receptor 2, VEGFR-2, FLK-1, KDR) (VEGFR2, VEGFR 2, Protein-tyrosine kinase receptor flk-1, Fetal liver kinase 1, Kinase NYK, FLK1, KDR, Kinase insert domain receptor, KRD1, Ly73, Protein tyrosine kinase receptor
Description:
Vascular endothelial growth factor receptor-2 (VEGFR-2), also known as fetal liver kinase 1 (FLK-1) in mouse and KDR in human, is a transmembrane tyrosine kinase that plays an important role in the development of the vascular system and the vascularization of a wide variety of tumors. Binding of VEGF to VEGFR-2 leads to tyrosine phosphorylation of the dimerized receptor and subsequent phosphorylation of SH2-containing intracellular signaling proteins, including phospholipase C, Src family tyrosine kinase and phosphatidylinositol 3-kinase (PI3K) adaptor molecules, SHC, NCK, and Ras GTPase-activating protein. VEGFR-2 is expressed in cultured human umbilical vein endothelial cells (HUVEC), Hela cells, WM 35 melanoma cells, WM 9 melanoma cells, as well as mouse NIH3T3, and Balb/c 3T3 cells.
In a single study of 115 paraffin-embedded cancer specimens analyzed by immunohistochemistry, VEGFR-2 expression was observed in bladder tumors, breast cancer, intestinal cancer and lung cancer, suggesting a direct role for VEGF as an autocrine growth factor in a variety of human cancers.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.1-1ug/ml
Western Blot: 15ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
~0.25mg/ml
Immunogen:
Synthetic peptide derived from the N-terminal region of the mouse vascular endothelial growth factor receptor-2 (VEGFR-2, FLK-1, KDR) protein.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Specificity:
Rrecognizes mouse VEGF Receptor-2. Identi es the ~230kD mature form of VEGFR-2. Reactivity is con rmed with mouse SVEC 410 cell lysates. Human reactivity is expected based on sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Botta, R.M., et al., Exp. Hematol. 28(12): 1501 (2000). 2. Chiang, M.K., et al., Growth Factors 12(1): 1-10 (1995). 3. Heidenreich, R., et al., Cancer Res. 60(21): 6142-6147 (2000). 4. Dayanir, V., et al., J. Biol. Chem. 276(21): 17,686-17,692 (2001). 5. Soker, S., et al., J. Biol. Chem. 271(10): 5761-5767 (1996). 6. Terman, B., et al., Growth Factors 11(3): 187-195 (1994). 7. Waltenberger, J., et al., J. Biol. Chem. 275(21): 15,905-15,911 (2000). 8. Tian, X., et al., Biochem. Biophys. Res. Commun. 286(3): 505-512 (2001).