		Technical Data
V2110-18J	Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr1175) (VEGFR2, KDR, Flk-1)

Description:
Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor transducing VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLC gamma as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).

Applications:
Suitable for use in Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:300
Immunofluorescence (IC): 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG	7H230	Supernatant

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

Synthetic peptide corresponding to residues surrounding Tyr1175 of human VEGF receptor 2 (KLH). MW: 230kD.

Purity:

Supernatant.

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.

Specificity:

Recognizes endogenous levels of human VEGFR-2 at ~230kD only when phosphorylated at tyrosine 1175. Does not crossreact with other phosphorylated receptor tyrosine kinases. May crossreact with VEGFR1. Species Crossreactivity: Mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Meyer, M. et al. (1999) EMBO J. 18, 363-374. 2. Dougher-Vermazen, M. et al. (1994) Biochem. Biophys. Res. Commun. 205, 728-738. 3. Kroll, J. and Waltenberger, J. (1997) J. Biol. Chem. 272, 32521-32527. 4. Takahashi, T. et al. (2001) EMBO J. 20, 2768-2778. 5. Holmqvist, K. et al. (2004) J. Biol. Chem. 279, 22267-22275. 6. Karkkainen, M.J. and Petrova, T. (2000) Oncogene 19, 5598-5605. 7. Rahimi, N. et al. (2000) J. Biol. Chem. 275, 16986-16992. 8. Claesson-Welsh, L. (2003) Biochem. Soc. Trans. 31, 20-24.