Technical Data
V2110-18J
Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr1175) (VEGFR2, KDR, Flk-1)
Description:
Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor transducing VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLC gamma as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).

Applications:
Suitable for use in Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:300
Immunofluorescence (IC): 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG7H230Supernatant
SizeStorageShippingSourceHost
100ul -20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues surrounding Tyr1175 of human VEGF receptor 2 (KLH). MW: 230kD.
Purity:
Supernatant.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes endogenous levels of human VEGFR-2 at ~230kD only when phosphorylated at tyrosine 1175. Does not crossreact with other phosphorylated receptor tyrosine kinases. May crossreact with VEGFR1. Species Crossreactivity: Mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Meyer, M. et al. (1999) EMBO J. 18, 363-374. 2. Dougher-Vermazen, M. et al. (1994) Biochem. Biophys. Res. Commun. 205, 728-738. 3. Kroll, J. and Waltenberger, J. (1997) J. Biol. Chem. 272, 32521-32527. 4. Takahashi, T. et al. (2001) EMBO J. 20, 2768-2778. 5. Holmqvist, K. et al. (2004) J. Biol. Chem. 279, 22267-22275. 6. Karkkainen, M.J. and Petrova, T. (2000) Oncogene 19, 5598-5605. 7. Rahimi, N. et al. (2000) J. Biol. Chem. 275, 16986-16992. 8. Claesson-Welsh, L. (2003) Biochem. Soc. Trans. 31, 20-24.