		Technical Data
V2110-18T2	Vascular Endothelial Growth Factor Receptor 2 (VEGF Receptor 2, VEGFR 2, VEGFR2, VEGFR-2, VEGFR, CD309 Antigen, Fetal Liver Kinase 1, FLK1, Kinase Insert Domain Receptor, KDR, KRD1, Ly73, Protein Tyrosine Kinase Receptor FLK1) (Biotin)

Description:
Vascular endothelial growth factor receptor-2 (VEGFR-2), also known as fetal liver kinase 1 (FLK-1) in mouse and KDR in human, is a transmembrane tyrosine kinase that plays an important role in the development of the vascular system and the vascularization of a wide variety of tumors. Binding of VEGF to VEGFR-2 leads to tyrosine phosphorylation of the dimerized receptor and subsequent phosphorylation of SH2-containing intracellular signaling proteins, including phospholipase C, Src family tyrosine kinase and phosphatidylinositol 3-kinase (PI3K) adaptor molecules, SHC, NCK, and Ras GTPase-activating protein. VEGFR-2 is expressed in cultured human umbilical vein endothelial cells (HUVEC), Hela cells, WM 35 melanoma cells, WM 9 melanoma cells, as well as mouse NIH3T3, and Balb/c 3T3 cells.

Applications:
Suitable for use in Flow Cytometry, ELISA, Immunohistochemistry and Cell Sorting. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 2-5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	9F482	Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml (after reconstitution)

Immunogen:

Recombinant protein corresponding to human soluble extracellular Vascular Endothelial Growth Factor Receptor 2 (110kD) native protein. Cellular Localization: Type I membrane protein

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a lyophilized powder from PBS, 2% sucrose, 0.02% sodium azide. Labeled with Biotin. Reconstitute with 50ul sterile ddH2O.

Specificity:

Recognizes native human Vascular Endothelial Growth Factor Receptor 2 at ~110kD. Species Crossreactivity: rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.