		Technical Data
V2110-20	Vascular Endothelial Growth Factor Receptor 3 (VEGF 3, Flt4, MYYN)

Description:
Applications:
Suitable for use in Immunohistochemistry, Westerm blot, and Immunocytochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: 1:100-1:400 High temperature antigen retrieval in 10mM citrate buffer (pH6.0) is strongly recommended. Blocking with 2% normal serum plus 1% BSA in PBS is suggested. Primary antibody should be diluted in PBS with 0.1% BSA only. Triton X-100 or excess blocking compounds is not recommended.
Western Blot: Membrane extract preparations and the liberal use of proteinase inhibitors is strongly recommended.
Optimal dilutions to be determined by the researcher.

Positive Control:
MCF7, SW480 and MDA-MB cell lines are positive controls for Immunocytochemistry.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

12 amino acid sequence corresponding to the N-terminus of human VEGF receptor 3.

Purity:

Purified IgG

Form

Supplied as a liquid in 0.1% BSA/PBS, pH 7.4, 0.0015% sodium azide.

Specificity:

Recognizes human VEGF Receptor 3 (FLT4).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.