Technical Data
Vascular Endothelial Growth Factor Receptor 3 (VEGF 3, Flt4, MYYN)
Suitable for use in Immunohistochemistry, Westerm blot, and Immunocytochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: 1:100-1:400 High temperature antigen retrieval in 10mM citrate buffer (pH6.0) is strongly recommended. Blocking with 2% normal serum plus 1% BSA in PBS is suggested. Primary antibody should be diluted in PBS with 0.1% BSA only. Triton X-100 or excess blocking compounds is not recommended.
Western Blot: Membrane extract preparations and the liberal use of proteinase inhibitors is strongly recommended.
Optimal dilutions to be determined by the researcher.

Positive Control:
MCF7, SW480 and MDA-MB cell lines are positive controls for Immunocytochemistry.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ug4C (-20C Glycerol)Blue IceHumanRabbit
12 amino acid sequence corresponding to the N-terminus of human VEGF receptor 3.
Purified IgG
Supplied as a liquid in 0.1% BSA/PBS, pH 7.4, 0.0015% sodium azide.
Recognizes human VEGF Receptor 3 (FLT4).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.