Technical Data
V2121-89S
Vibrio cholerae, Cholera Toxin
Description:
Vibrio cholerae (also Kommabacillus) is a gram negative, facultative, comma-shaped bacterium with a polar flagellum that causes cholera in humans. V. cholerae and other species of the genus Vibrio belong to the gamma subdivision of the Proteobacteria.

Applications:
Suitable for use in ELISA, Western Blot, Indirect Immunofluorescence and Particle Agglutination. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Source:
Ascites

Biological Activity:
Evaluated with two panels of microorganisms normally present in stool samples. One panel include 72 potential antigenically related bacterial strains, the second panel included pathogenic bacterial strains involved in diarrhea cases. Reacts with cholera antigens in Western Blot.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12A28Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouse
Concentration:
~0.25mg/ml
Immunogen:
Vibrio cholerae 01, serotype Ogawa, biotype ELT inactivated at 56°C for 30 minutes.
Purity:
Purified
Form
Supplied as a liquid in 0.1M Tris-HCl, pH 7.5.
Specificity:
Recognizes LPS epitopes of Vibrio cholerae antigens present in the bacterial cell wall.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Castillo L., Castillo D., Silva W., Zapapta L., Reiod M., Ulloa MT., Maldonado A., Valenzuela ME., Bustos R., Tamplin ML., Martinez MA., De Ioannes AE., Jamett A., Yudelevich A., Becker MI., Development of highly specific monoclonal antibodies for the diagnosis of Vibrio cholerae 0, Hybridoma 14(3) 277-278 (1995).