Technical Data
V2122-01C
Vimentin (FLJ36605, VIM)
Description:
Human vimentin is a 53,521 dalton protein (466aa) expressed in many cell types of mesodermal origin, and is one of the intermediate filament types that form the cytoskeleton. In the embryonic nervous system, however, vimentin is expressed by migrating neural crest cells forming the PNS, and by neural stem cells of the CNS. In some forms of glioblastoma, vimentin is re-expressed by the tumor cells and serves as a general indicator of their undifferentiated state. Immunohistochemical staining for Vimentin is also characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:250-1:500
Immunohistochemistry: 1:100-1:200
Immunocytochemistry: 1:100-1:200
IOptimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1906: IgY, Chicken Pab Rb x Ch
I1906-10: IgY, Chicken (HRP) Rb x Ch
I1906-12: IgY, Chicken (FITC) Rb x Ch
I1906-14: IgY, Chicken (AP) Rb x Ch

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgYAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanChicken
Concentration:
~0.1mg/ml
Immunogen:
Peptide corresponding to a region of the Vimentin gene product shared between the mouse (NP_035831, NCBI) and human (NP_003371, NCBI) gene products.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity:
Recognizes human Vimentin. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.