Technical Data
V2122-09
Vimentin (FLJ36605, VIM)
Description:
Vimentin (57kD) is the intermediate filament protein (IFP) of mesenchymal cells. It is the only IFP, which deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type.
Desmin (53kD) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous.
Coexpression of vimentin and desmin has been observed in rhabdomyosarcomas and leiomyosarcomas.

Applications:
Suitable for use in Immunohistochemistry, Flow Cytometry, Immunocytochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50-1:100
Immunohistochemistry (Frozen): 1:50-1:100 using ABC as detection reagent.
Western Blot: 1:50-1:500
Optimum dilutions to be determined by researcher.

Hybridoma:
Sp2/0-Ag14 mouse myeloma cells with spleen cells of Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG14A84Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Cytoskeletal Vimentin extract from calf lens.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, before the addition of glycerol to 40%. No stabilizing proteins added.
Specificity:
Recognizes 57kD protein corresponding to vimentin in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. Species Crossreactivity: human, mouse, rat, hamster, chicken, goat, canine and monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
USBio product reference: 1. Cheng et al. Stem Cell Research & Therapy (2010) 1:6-13. 1. Ramaekers F, Huijsmans A, Schaart G, Moesker O, Vooijs P - Tissue distribution of keratin 7 as monitored by a monoclonal antibody. Exp Cell Res 170: 235-249 (1987). 2. Pieper FR, Schaart G, Krimpenfort PJ, Henderik JB, Moshage HJ, Van de Kemp A, Ramaekers FCS, Berns A, Bloemendal H - Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells. J Cell Biol 108: 1009-1024 (1989). 3. Viebahn C, Lane EB, Ramaekers FCS - Keratin and vimentin expression in early organogenesis of the rabbit embryo. Cell Tissue Res 253: 553-562 (1988). 4. Raats JMH, Pieper FR, Vree Egberts WTM, Verrijp KN, Ramaekers FCS, Bloemendal H - Assembly of amino-terminal deleted desmin and vimentin-free cells. J Cell Biol 111: 1971-1985 (1990). 5. Ramaekers FCS, Van Niekerk CC, Poels LG, Schaafsma E, Huijsmans A, Robben H, Schaart G, Vooijs GP - Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 136 : 641-655 (1990).