Technical Data
V2129
1,25 Dihydroxy Vitamin D3 (Calcitriol, 1,25-dihydroxycholecalciferol)
Description:
Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:25,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1ID1,25A Affinity Purified
SizeStorageShippingSourceHost
10ug-20CBlue IceMouse
Concentration:
~1mg/ml (after reconstutution)
Immunogen:
1,25(OH)2 D3 3-Hemisuccinate-BSA
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from 50mM TRIS, pH 7.4. Reconstitute with 10ul sterile ddH2O.
Specificity:
Recognizes 1,25-Dihydroxy-Vitamin D3. Crossreactivity: 1,25(OH)2 D3(Calcitriol): 100%; 1,25(OH)2 D2(Ergocalcitriol): 100%, 24,25(OH)2 D3(24,25-dihydroxycholecaciferol): 20.5%, 25-(OH) D3(Calcidiol): 4.4%, 25-(OH) D2(Ergocalcidiol): 2.3%, D3(Cholecalciferol): 0.3%, D2 (Ergocalciferol) 1.0%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.