		Technical Data
V2129-10	25-OH Vitamin D3 (Calcitriol (1,25-Dihydroxyvitamin D3, 1,25-dihydroxycholecalciferol)

Description:
Calcitriol, also called 1,25-dihydroxycholecalciferol or 1,25-dihydroxyvitamin D3, is the hormonally active form of vitamin D with three hydroxyl groups (abbreviated 1,25-(OH)2D3 or simply 1,25(OH)2D). It increases the level of calcium (Ca2+) in the blood by increasing the uptake of calcium from the gut into the blood, decreasing the transfer of calcium from blood to the urine by the kidney, and increasing the release of calcium into the blood from bone.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
ELISA: 1:10,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	ID1,25B	Affinity Purified

Size		Storage	Shipping	Source	Host
10ug		-20°C	Blue Ice		Mouse

Concentration:

~1mg/ml (after reconstitution)

Immunogen:

25-OH D3 3-Hemisuccinate-BSA

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 2% BSA. Reconstitute with 10ul sterile dH2O.

Specificity:

Recognizes 25-(OH) Vitamin D3 (100%). Crossreactivity: 24,25(OH)2 D3: 80%; 1,25(OH)2 D3: 20%; 1,25(OH)2 D2: 0%

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.