VDR is a member of the super family of steroid hormone receptors. It is known to play a role in cellular growth and differentiation, calcium and phosphorus metabolism and bone remodeling. Human breast carcinomas, leukemias, and prostatic carcinoma cells express VDR, which can modify their growth and differentiation. Human breast carcinomas, leukemias, and prostatic carcinoma cells express VDR which can modify their growth and differentiation.
Suitable for use in Gel Supershift Assays, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.
Immunohistochemistry (Paraffin and frozen sections): 5ug/ml
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Partially purified chicken intestinal cytoplasmic VDR (vitamin D3 receptor).|
|Supplied as a liquid in 0.05% sodium azide.|
|Recognizes the occupied and unoccupied forms of chicken VDR. Recognizes an epitope between aa 89 and 105 of human VDR. In avians detects bands of ~58kD and ~60kD by Western blot, while in mammalian species the VDRs appear as a single band within the ~52-55kD range. Does not cross-react with ER (estrogen receptor) or GR (glucocorticoid receptor). Species Crossreactivity: human, mouse, rat, hamster, amphibian and fish.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Development of hybridomas secreting monoclonal antibodies to the chicken intestinal 1 alpha,25-dihydroxyvitamin D3 receptor: J.W. Pike, et al.; PNAS 79, 7719 (1982) 2. Serum and monoclonal antibodies against the chick intestinal receptor for 1,25-dihydroxyvitamin D3. Generation by a preparation enriched in a 64,000-dalton protein: J.W. Pike, et al.; J. Biol. Chem. 258, 1289 (1983) 3. ,Immunocytochemical localization of the 1,25-dihydroxyvitamin D3 receptor in target cells: T.L. Clemens, et al.; Endocrinology 122, 1224 (1988) 4. Molecular biology of the vitamin D hormone: M.R. Haussler, et al.; Recent Progr. Horm. Res. 44, 263 (1988) 5. Immunohistochemical detection of 1,25-dihydroxyvitamin D3 receptors and estrogen receptors by monoclonal antibodies: comparison of four immunoperoxidase methods: P. Milde, et al.; J. Histochem. Cytochem. 37, 1609 (1989) 6. Baculovirus-mediated expression of the human vitamin D receptor. Functional characterization, vitamin D response element interactions, and evidence for a receptor auxiliary factor: P.N. MacDonald, et al.; J. Biol. Chem. 266, 18808 (1991)|