Technical Data
V2130-20
Vitamin D Binding Protein (VDBP, VDB, DBP, DBP/GC, Gc Globulin, Group Specific Component, Group-specific Component, GC, hDBP, Vitamin D Binding alpha Globulin, VDBG)
Description:
Vitamin-D binding protein (VDBP, DBP, Gc) is a multifunctional plasma protein that has many physiologically important functions, ranging from transporting vitamin D3 metabolites, binding and sequestering globular actin and binding fatty acids to functioning in the immune system. It is a member of a multigene family including alpha-fetoprotein and albumin. It is a serum glycoprotein that reversibly binds and transports vitamin D and its metabolites to target cells. VDBP has a triple domain modular structure with extensive disulfide bonding that is characteristic of this protein family. VDBP receptors are constitutively expressed by malignant B-cells and in a transitory form by normal B-lymphocytes undergoing mitogen-induced activation (1-4).

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:40
Western Blot: 1:5000- 1:20,000
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 



Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9E636Supernatant
SizeStorageShippingSourceHost
100ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues within human VDBP.
Purity:
Supernatant
Form
Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Specificity:
Recognizes human Vitamin D binding protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cooke NE, et al. J Clin Invest 76(6):2420-4, 1985 2. Verboven C, et al. Nat. Struct Biol 9(2):131-6, 2002 3. Zhang J, et al. The Journal of biological chemistry 279(51):53282-53287, 2004 4. Esteban C, et al. J Biol Chem. 267(14):10177-83, 1992