Technical Data
V2150-51
Vitellogenin (carp)
Description:
Vitellogenin (Vg) is an egg yolk precursor protein expressed in female fish, dormant in male fish and female insects, but in the presence of estrogenic endocrine disrupting chemicals (EDCs), males can express the Vg gene in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDCs.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:100-1:1000
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1k6D833Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceMouse
Concentration:
Not determined
Immunogen:
Vitellogenin from plasma of 17 -estradiol treated striped bass (Morone saxatilis), brown bullhead (Ameiurus nebulosus), and common carp (Cyprinus carpio).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Specificity:
Recognizes vitellogenin from common carp (Cyprinus carpio). Cross-reacts with vitellogenin from fathead minnow (Pimephales promelas), zebrafish (Danio rerio), goldfish (Carassiu auratus), mullet (Mugil sp.), pinfish (Lagodon rhomboides), and sucker (Catastomidea sp.). Does not cross-react with vitellogenin from striped bass (Morone saxatilis) or brown bullhead (lctalurus nebulosus).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.