Vitellogenin (Vg) is an egg yolk precursor protein expressed in female fish, dormant in male fish and female insects, but in the presence of estrogenic endocrine disrupting chemicals (EDCs), males can express the Vg gene in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDCs.
Suitable for use in ELISA and Western Blot. Other applications not tested.
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
| Not determined|
|Vitellogenin from plasma of 17beta-estradiol treated killifish (Fundulus heteroclitus), white perch (Morone americanus), bowfish (Xiphias gladius) and egg yolk from swordfish.|
|Purified by immunoaffinity chromatography.|
|Supplied as a liquid in PBS, pH 7.4, 0.15M sodium chloride, 0.09% sodium azide, 1% BSA.|
|Recognizes vitellogenin from killifish (Fundulus heteroclitus) and white perch (Morone americanus). Cossreacts with vitellogenin from a variety of different species including common carp (Cyprinus carpio), wrasse (Ctenolabrus rupestris), tilapia (Oreochromis niloticus), halibut (Hippoglossus hippoglossus) and sheepshead minnow (Cyprinodon variegatus). Does not react with vitellogenin from bowfin (Amia calva) or with egg yolk from swordfish (Xiphias gladius). |
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|