Technical Data
Vitellogenin (killifish)
Vitellogenin (Vg) is an egg yolk precursor protein expressed in female fish, dormant in male fish and female insects, but in the presence of estrogenic endocrine disrupting chemicals (EDCs), males can express the Vg gene in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDCs.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:100-1:1000
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG1,k6D836Affinity Purified
100ul-20CBlue IceMouse
Not Determined
Vitellogenin from plasma of 17beta-estradiol treated killifish (Fundulus heteroclitus), white perch (Morone americanus), bowfin (Amia calva). Egg yolk from swordfish (Xiphias gladius).
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide
Recognizes vitellogenin from killifish (Fundulus heteroclitus) and white perch (Morone americanus). Cossreacts with vitellogenin from a variety of different species including common carp (Cyprinus carpio), wrasse (Ctenolabrus rupestris), tilapia (Oreochromis niloticus), halibut (Hippoglossus hippoglossus) and sheepshead minnow (Cyprinodon variegatus). Does not react with vitellogenin from bowfin (Amia calva) or with egg yolk from swordfish (Xiphias gladius).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.