Technical Data
V2150-56
Vitellogenin (stickleback)
Description:
Vitellogenin (Vg) is an egg yolk precursor protein expressed in female fish, dormant in male fish and female insects, but in the presence of estrogenic endocrine disrupting chemicals (EDCs), males can express the Vg gene in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDCs.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:50-1:500
Western Blot: 1:50-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgG2b,kAffinity Purified
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceRabbit
Concentration:
Not determined
Immunogen:
Vitellogenin from whole body homogenate of 17b-estradiol treated three-spined stickleback.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 12.3mM sodium azide, and 1% BSA, before tha addition of glycerol to 40%.
Specificity:
Recognizes vitellogenin from stickleback (Gasterosteus aculeatus). Species Crossreactivity: Rainbow trout (Oncorhynchus mykiss), tilapia (Oreochromis niloticus), sheepshead minnow (Cyprinodon variegatus), cod (Gadus morhua), turbot (Scophthalmus maximus) and ballan wrasse (Labrus bergylta).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.