Technical Data
V2150-59
Vitellogenin (zebrafish)
Description:
Vitellogenin (Vg) is an egg yolk precursor protein expressed in female fish, dormant in male fish and female insects, but in the presence of estrogenic endocrine disrupting chemicals (EDCs), males can express the Vg gene in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDCs.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1,000
Western Blot: 1:100-1:1,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b,kJE-2A6Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceZebrafishMouse
Concentration:
Immunogen:
Vitellogenin from whole body homogenates of 17 -estradiol treated zebrafish.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 12mM sodium azide.
Specificity:
Recognizes vitellogenin from zebrafish. Does not cross-react with vitellogenin from carp, fathead minnow, or roach.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.