Technical Data
V2150-59D
Vitellogenin (zebrafish)
Description:
Vitellogenin (Vg) is an egg yolk precursor protein expressed in female fish, dormant in male fish and female insects, but in the presence of estrogenic endocrine disrupting chemicals (EDCs), males can express the Vg gene in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDCs.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:100-1:1000.
Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating.
Western Blot: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k6D844Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceZebrafishMouse
Concentration:
Immunogen:
Vitellogenin from whole body homogenate of 17beta-estradiol treated zebrafish.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 12mM sodium azide.
Specificity:
Recognizes vitellogenin in whole body homogenate samples from zebrafish. Cross-reacts with vitellogenin from carp, fathead minnow, and roach.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.