Technical Data
V2150-59H
Vitellogenin (zebrafish)
Description:
Vitellogenin (Vg) is an egg yolk precursor protein expressed in female fish, dormant in male fish and female insects, but in the presence of estrogenic endocrine disrupting chemicals (EDCs), males can express the Vg gene in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDCs.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:100-1:1000. Not recommended for coating due to presence of BSA.
Western Blot: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k6D845Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceZebrafishMouse
Concentration:
Not determined
Immunogen:
Vitellogenin from whole body homogenate of 17beta-estradiol treated zebrafish (Danio rerio).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 12mM sodium azide.
Specificity:
Recognizes vitellogenin in whole body homogenate samples of zebrafish (Danio rerio). Crossreactivity: Vitellogenin from other cyprinid species, including carp (Cyprinus carpio), fathead minnow (Pimephales promelas) and roach (Rutilus rutilus).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.