Technical Data
Vitronectin (Vitronectin V10 Subunit, Vitronectin V65 Subunit, VN, VNT, VTN, Complement S Protein, Epibolin, S Protein, S-Protein, Serum Spreading Factor, Somatomedin B, Somatomedin-B, V75)
Vitronectin is an abundant glycoprotein present in plasma and the extracellular matrix of most tissues. Though the precise function of VN in vivo is unknown, it has been implicated as a participant in diverse biological processes, including cell attachment and spreading, complement activation and regulation of hemostasis. The major site of synthesis appears to be the liver, though VN is also found in the brain at an early stage of mouse organogenesis, suggesting that it may play an important role in mouse development. Genetic deficiency of VN has not been reported in humans or in other high organisms.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:100,000
Optimal dilutions to be determined by the researcher.

Solubility: >10mg/ml

Ultraviolet: Absorbance (280nm) = 11.56 0.1% = 1.36

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
1ml-20CBlue IceHumanRabbit
Vitronectin purified from human plasma using non-denaturing chromatography.
98% pure (SDS-PAGE)
Supplied as a liquid in 0.1M HEPES, 0.1M sodium chloride, 1mM EDTA, pH 7.4.
Reacts with both native monomeric and polymeric human vitronectin in various tissues and plasma.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.