Technical Data
V2200-06B
Vitronectin (Vitronectin V10 Subunit, Vitronectin V65 Subunit, VN, VNT, VTN, Complement S Protein, Epibolin, S Protein, S-Protein, Serum Spreading Factor, Somatomedin B, Somatomedin-B, V75)
Description:
Vitronectin is a 71kD secreted glycoprotein produced by the liver and tumor cells. In blood, Vitronectin is called serum spreading factor. In the extracellular matrix, its function is determined by binding partners such as PAI-1, complement factors, integrins (notably alpha v beta 3) and thrombin. The 459 aa mature mouse Vitronectin shows 74% amino acid identity with human Vitronectin and contains somatomedin B-like and hemopexin-like domains, an RGD motif, a basic heparin-binding domain and sulfated tyrosines. Unbound Vitronectin is a monomer that may be cleaved to form a dimer of 65kD and 10kD components.

Applications:
Suitable for use in Immunohistochemistry and ELISA. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 10ug/ml detects Vitronectin on mouse fetal brain tissue sections. Sections were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted V2200-06B overnight at 4 C followed by Rhodamine Red-coupled anti-rat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS, 0.1% BSA.
Direct ELISA: 0.5-1ug/ml. The detection limit is ~3ng/well.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a7H111Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Recombinant corresponding to aa20-478 from mouse Vitronectin expressed in NSO cells (P29788).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity:
Recognizes mouse Vitronectin. Does not crossreact with recombinant human Vitronectin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.