Technical Data
V2200-15
Vitronectin (Vitronectin V10 Subunit, Vitronectin V65 Subunit, VN, VNT, VTN, Complement S Protein, Epibolin, S Protein, S-Protein, Serum Spreading Factor, Somatomedin B, Somatomedin-B, V75)
Description:
Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin is circulating as a mixture of both 75kD and 65kD forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognises fibronectin (2).

Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32000
Western Blot: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b,kHV2Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human vitronectin purified from plasma by heparin-affinity chromatography.
Purity:
Purified by Protein A/G affinity chromatography from culture supernatant.
Form
Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity:
Highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). This antibody is human-specific (no reactivity with cat, dog, cow, sheep, goat, pig, rabbit, horse). The epitope is located in the connecting region. Binding can be competed selectively by a peptide comprising aa 121-133. Reactivity: Can be used to purify vitronectin from human plasma by affinity chromatography. It can also be used to quantitatively affinity-deplete human plasma or serum of vitronectin. It partially denatures vitronectin upon antibody binding. It binds equally well to native and denatured vitronectin and can be used to quantitate vitronectin in human plasma in a sandwich ELISA with clone 6F211. It does not interfere with the binding of any known vitronectin ligands. Also binds to vitronectin in ELISA when vitronectin is coated directly onto the microtiter well.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Morris CA, Underwood PA, Bean PA, Sheehan M, Charlesworth JA (1994) Relative topography of biologically active domains of human vitronectin. Evidence from monoclonal antibody epitope and denaturation studies. J Biol Chem 269:23845-23852.
2. Underwood PA, Kirkpatrick A, Mitchell SM (2002) New insights into heparin binding to vitronectin: studies with monoclonal antibodies. Biochem J 365:57-67.