Technical Data
Media
Vogel and Johnson Agar (Powder)
V2500
Microbiological Grade
Storage RT    Shipping RT
Component (g/L)
Enzymatic Digest of Casein10
Yeast Extract.5
Mannitol10
Dipotassium Phosphate5
Lithium Chloride5
Glycine10
Phenol Red0.025
Agar15
Supplement: Tellurite Solution (1%)
USP medium for detection of coagulase positive Staphylococcus aureus.

Coagulase-positive strains of S. aureus reduce tellurite and form black colonies on the medium. These strains typically ferment mannitol and exhibit yellow halos around black colonies. Most organisms other than coagulase-positive staphylococci are inhibited during the first 24 hours of incubation.


1.Suspend 60 g of the medium in one liter of purified water. 2 Heat with frequent agitation and boil for one minute to completely dissolve the medium. 3.Autoclave at 121C for 15 minutes.

4. After cooling to 45 - 50C add 20 mL of a sterile 1% Potassium Tellurite Solution. 5. Mix thoroughly before dispensing.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.