Technical Data
V2700-08
Von Willebrand Factor (vWF, Factor VIII Related Antigen)
Description:
Von Willebrand Factor is synthesized by endothelial cells, causing adhesion of platelets to injured vessel walls and functions as a carrier and stabilizer for coagulation of Factor VIII. It is one of the most widely used endothelial cell markers for studying angiogenesis/neovascularization.

Applications:
Suitable for use in ELISA, RIA, Immunofluorescence, Western Blot, Immunocytochemistry, Immunohistochemistry and Immunoperoxidase staining. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000. Detected vWF in human placenta lysates.
Indirect Immunoperoxidase Staining: 1:50
Immunohistochemistry: Frozen and formalin fixed sections.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-02C: IgG, H&L (HRP) Pab Dk xMo
I1904-03C: IgG, H&L (X-Adsorbed) (HRP) Pab Dk xMo

pI: 6.55

Source: Ascites

Control: Tonsil

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q2134Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Purified human Factor VIII complex.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide, 50% glycerol.
Specificity:
Recognizes human von Willebrand Factor or Factor VIII related antigen at ~310kD in both plasma and tissues.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Francis, S.E., et al., Pathology 17: 579-585 (1985). 2. Exner, T., et al., Pathology 16: 356 (1984). 3. Francis, S., et al., Some studies with monoclonal antibodies to Human Factor VIII. Proceedings of the Haematology Society of Australia held in Perth, 1984.