		Technical Data
V2700-08	Von Willebrand Factor (vWF, Factor VIII Related Antigen)

Description:
Von Willebrand Factor is synthesized by endothelial cells, causing adhesion of platelets to injured vessel walls and functions as a carrier and stabilizer for coagulation of Factor VIII. It is one of the most widely used endothelial cell markers for studying angiogenesis/neovascularization.

Applications:
Suitable for use in ELISA, RIA, Immunofluorescence, Western Blot, Immunocytochemistry, Immunohistochemistry and Immunoperoxidase staining. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000. Detected vWF in human placenta lysates.
Indirect Immunoperoxidase Staining: 1:50
Immunohistochemistry: Frozen and formalin fixed sections.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-02C: IgG, H&L (HRP) Pab Dk xMo
I1904-03C: IgG, H&L (X-Adsorbed) (HRP) Pab Dk xMo

pI: 6.55

Source: Ascites

Control: Tonsil

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	2Q2134	Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.1mg/ml

Immunogen:

Purified human Factor VIII complex.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide, 50% glycerol.

Specificity:

Recognizes human von Willebrand Factor or Factor VIII related antigen at ~310kD in both plasma and tissues.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Francis, S.E., et al., Pathology 17: 579-585 (1985). 2. Exner, T., et al., Pathology 16: 356 (1984). 3. Francis, S., et al., Some studies with monoclonal antibodies to Human Factor VIII. Proceedings of the Haematology Society of Australia held in Perth, 1984.