Technical Data
V4990
VspI
1000U
5000U
Molecular Biology Storage: -20CShipping: Blue Ice
5'-A T^T A A T-3'
3'-T A A T^T A-5'

Enzyme Properties:
Stability during Prolonged Incubation: A minimum of 0.1 units of enzyme is required for complete digestion of 1ug of DNA in 16 hours at 37C.

Thermal Inactivation: Enzyme is inactivated by incubation at 65C for 20min.

Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.

Compatible Ends: Csp6I, MaeI, NdeI, Tru1I

Number of Recognition Sites in DNA:
Lambda: 17
PhiX174: 2
M13mp18/19: 7
pBR322: 1
pUC18/19: 3
pUC57: 3
pTZ19R/U: 3
pBluescriptIIKS(-/+): 3
pBluescriptIISK(-/+): 3
pACYC177: 2
pACYC184: 1

Supplied With: Provided to simply buffer selection.
R1625: Restriction Enzyme Buffer A, 10X.
R1625-03: Restriction Enzyme Buffer D, 10X.

Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.

Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Vibrio species

Concentration: 10u/ul

Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of assay buffer.

Quality Control:
Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 320-fold overdigestion (20u/ug lambda DNA x 16 hours) with VspI.

Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with VspI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.2uM. More than 95% of these can be recut.

Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.