Technical Data
W0478-01
WC5 (FITC)
Description:
WC5 is expressed by B cells and activated T cells.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG19i132Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceBovineMouse
Concentration:
~0.1mg/ml
Immunogen:
Whole cell preparation from bovine cell line BL20.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes bovine WC5 at ~47kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Naessens, J. & Howard, C.J. (1991) Individual antigens of cattle. Monoclonal antibodies reacting with bovine B cells (BoWC3, BoWC4 and BoWC5). Vet. Immunol. Immunopathol. 27: 77-85. 2. Howard, C.J. et al. (1993) Summary of workshop findings for cattle (Tables 1 and 2). Vet Immunol. Immunopathol. 39:25-47.