Technical Data
W0481
WC8
Description:
WC8, a cell surface antigen, is a 160kD protein which is expressed by activated bovine T cells. WC8 is not present on resting lymphocytes, but can be induced on T cells following activation by alloantigens or lectins (1).

Applications:
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19i133Affinity Purified
SizeStorageShippingSourceHost
250ug-20°CBlue IceBovineMouse
Concentration:
~1mg/ml
Immunogen:
Con A stimulated bovine lymphocytes.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH7.4, 0.09 sodium azide.
Specificity:
Recognizes the bovine WC8 cell surface antigen.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Nthale, J.M. et al. (1993) Characterization of a late activation antigen defined by monoclonal antibodies of cluster BoWC8 (TC23). Vet. Immunol. Immunopathol. 39: 201-208. 2. Howard, C.J. et al. (1993) Summary of workshop findings for cattle (Tables 1 and 2). Vet. Immunol. Immunopathol. 39: 25-48.