Technical Data
W0481-74A
WC1 (Antigen WC1.1, CD163L1, CD163 Molecule-like 1)
Description:
WC1 expression appears to be heterogeneous and antibodies of this cluster show differing reaction patterns. This clone immunoprecipitates a 215 kD molecule from bovine cells, and also recognizes the swine homologue of WC1, which is a 180kD molecule. In pigs the 180kD molecule is expressed by a gamma/delta TCR positive T-cell population that is also CD2-, CD4-, CD8-.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a10F580Affinity Purified
SizeStorageShippingSourceHost
250ug-20CBlue IceBovineMouse
Concentration:
~1mg/ml
Immunogen:
Con A stimulated bovine lymphocytes.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes bovine WC1 cell surface antigen, expressed by a population of gamma/delta TCR expression cells which lack CD2, CD4 and CD8 but express CD3. Species Crossreactivity: porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Carr, M.M. et al. (1994) Expression on porcine gamma/delta lymphocytes of a phylogenetically conserved surface antigen previously restricted in expression to ruminant gamma/delta T lymphocytes. Immunology 81: 36-40. 2. Howard, C.J. and Naessens, J. (1993) Summary of workshop findings for cattle Vet. Immunol. Immunopathol. 39: 25-48.