		Technical Data
W0481-74B	WC1 (T19)

Description:
Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:25-1:50; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
NS-1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	10F582	Affinity Purified

Size		Storage	Shipping	Source	Host
250ug		-20°C	Blue Ice	Sheep	Mouse

Concentration:

~1mg/ml

Immunogen:

Ovine thymocytes.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Specificity:

Recognizes sheep WC1. Species Crossreactivity: bovine, goat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Mackay, C. et al. (1986) Three distinct subpopulations of sheep T lymphocytes. Eur. J. Immunol. 16: 19 - 25. 2. Mackay, C. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset. Cell. Immunol. 110: 46 -55.