Technical Data
W0481-74D
WC1 (Antigen WC1.1, CD163L1, CD163 Molecule-like 1)
Description:
Bovine WC1 is a 215/300kD antigen expressed on the majority of gamma/delta T lymphocytes. These cells also express low levels of CD5, but are negative for other B and T cell markers.

The WC1 antigen is reported to be involved in a number of processes, including activation of gamma/delta T cells and the development of a Th1-biased acquired immune response.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry (frozen and paraffin), and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10; 25ul labels 10e6 cells in 100ul
Immunohistochemistry (frozen): 1:10
Immunohistochemistry (paraffin): Requires protein digestion pre-treatment of paraffin sections e.g. protease. Overnight incubation is recommended.
Optimal dilutions to be determined by the researcher.

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

TypeIsotypeCloneGrade
MabIgG2a10F583Supernatant
SizeStorageShippingSourceHost
2ml-20CBlue IceBovineMouse
Concentration:
As reported
Immunogen:
Bovine lymphocytes.
Purity:
Supernatant
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes bovine WC1. Species Crossreactivity: sheep, goat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Howard, C. J. et al. (1989) In vivo depletion of BoT4 (CD4) and of non-T4/T8 lymphocyte subsets in cattle with monoclonal antibodies. Eur. J. Immunol. 19: 757-764. 2. Clevers, H. et al. (1990) Identification of a bovine surface antigen uniquely expressed on CD4- CD8- T cell receptor gamma/delta+ T lymphocytes. Eur. J. Immunol. 20: 809-817. 3. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques. Vet. Immunol. Immunopathol. 71: 321-334. 4. Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leukcocyte surface antigens of human and 11 animal species. Vet. Immunol. Immunopathol. 64: 1-13. 5. Winkler, M. T. C. et al. (2000) Persistence and Reactivation of Bovine Herpesvirus 1 in the Tonsils of Latently Infected Calves. J. Virol. 74: 5337-5346.