Technical Data
W0481-74F
WC1 (T19) (FITC)
Description:
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
NS-1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG110F582Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceSheepMouse
Concentration:
~0.1mg/ml
Immunogen:
Ovine thymocytes.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes the ovine WC1 cell surface antigen, a 215kD glycoprotein which is expressed by a population of gamma/delta T lymphocytes. Species Crossreactivity: bovine, goat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mackay, C. et al. (1986) Three distinct subpopulations of sheep T lymphocytes. Eur. J. Immunol. 16: 19 - 25. 2. Mackay, C. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset. Cell. Immunol. 110: 46 -55.