Technical Data
W0481-74G
WC1 (Antigen WC1.1, CD163L1, CD163 Molecule-like 1) (FITC)
Description:
Bovine WC1 is a 215/300kD antigen expressed on the majority of gamma/delta T lymphocytes. These cells also express low levels of CD5, but are negative for other B and T cell markers.

The WC1 antigen is reported to be involved in a number of processes, including activation of gamma/delta T cells and the development of a Th1-biased acquired immune response.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat-1:10; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2a10F583Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceBovineMouse
Concentration:
~0.1mg/ml
Immunogen:
Bovine lymphocytes.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes bovine WC1. Species Crossreactivity: sheep, goat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Howard, C. J. et al. (1989) In vivo depletion of BoT4 (CD4) and of non-T4/T8 lymphocyte subsets in cattle with monoclonal antibodies. Eur. J. Immunol. 19: 757-764. 2. Clevers, H. et al. (1990) Identification of a bovine surface antigen uniquely expressed on CD4- CD8- T cell receptor gamma/delta+ T lymphocytes. Eur. J. Immunol. 20: 809-817. 3. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques. Vet. Immunol. Immunopathol. 71: 321-334. 4. Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leukcocyte surface antigens of human and 11 animal species. Vet. Immunol. Immunopathol. 64: 1-13. 5. Winkler, M. T. C. et al. (2000) Persistence and Reactivation of Bovine Herpesvirus 1 in the Tonsils of Latently Infected Calves. J. Virol. 74: 5337-5346.