Description:
WC9 is expressed on bovine monocytes, platelets, granulocytes, peripheral B lymphocytes and a subset of T lymphocytes.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:10-1:50 10ul labels 1x10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.
Hybridoma:
P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1 | 9E66 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 250ug | | -20°C | Blue Ice | Bovine | Mouse |
|
| Concentration: |
| As reported |
| Immunogen: |
| Bovine WC9 cell surface antigen. |
| Purity: |
| Purified by Protein G Chromatography. |
| Form |
| Supplied as a liquid in PBS, pH7.4, 0.09% sodium azide. |
| Specificity: |
| Recognizes the bovine WC9 cell surface antigen. Species Crossreactivity: sheep. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Naessens, J. et al. (1993) Biochemical characterization of three non-lineage antigens defined by workshop antibodies. Vet. Immunol. Immunopathol. 39: 217-223. 2. Menge, C. et al. (2004) Bovine Ileal Intraepithelial Lymphocytes Represent Target Cells for Shiga Toxin 1 from Escherichia coli. Infect. Immun. 72: 1896–1905. 3. Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants. Vet. Immunol. Immunopathol. 39: 283-290. 4. Howard, C.J. et al. (1993) Summary of workshop findings for cattle (Tables 1 and 2). Vet Immunol. Immunopathol. 39:25-48.
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