WC9 is expressed on bovine monocytes, platelets, granulocytes, peripheral B lymphocytes and a subset of T lymphocytes.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
|100 Tests||4°C Do Not Freeze||Blue Ice||Bovine||Mouse|
| As reported|
|Purified by Protein G affinity chromatography. |
|Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.|
|Recognizes the bovine WC9 cell surface antigen. Species Crossreactivity: sheep.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Naessens, J. et al. (1993) Biochemical characterization of three non-lineage antigens defined by workshop antibodies. Vet. Immunol. Immunopathol. 39: 217-223. 2. Menge, C. et al. (2004) Bovine Ileal Intraepithelial Lymphocytes Represent Target Cells for Shiga Toxin 1 from Escherichia coli. Infect. Immun. 72: 1896-1905. 3. Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants. Vet. Immunol. Immunopathol. 39: 283-290. 4. Howard, C.J. et al. (1993) Summary of workshop findings for cattle (Tables 1 and 2). Vet Immunol. Immunopathol. 39:25-48.|