Technical Data
W0482-01A
WC9 (PE)
Description:
WC9 is expressed on bovine monocytes, platelets, granulocytes, peripheral B lymphocytes and a subset of T lymphocytes.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG19E66Affinity Purified
SizeStorageShippingSourceHost
100Tests4C Do Not FreezeBlue IceBovineMouse
Concentration:
As reported
Immunogen:
Bovine WC9.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes the bovine WC9 cell surface antigen. Species Crossreactivity: sheep.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Naessens, J. et al. (1993) Biochemical characterization of three non-lineage antigens defined by workshop antibodies. Vet. Immunol. Immunopathol. 39: 217-223. 2. Menge, C. et al. (2004) Bovine Ileal Intraepithelial Lymphocytes Represent Target Cells for Shiga Toxin 1 from Escherichia coli. Infect. Immun. 72: 1896-1905. 3. Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants. Vet. Immunol. Immunopathol. 39: 283-290. 4. Howard, C.J. et al. (1993) Summary of workshop findings for cattle (Tables 1 and 2). Vet Immunol. Immunopathol. 39:25-48.