Description:
WC14 is expressed on monocytes and granulocytes, and a subset of activated T cells.
Applications:
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Flow Cytometry: Neat-1:5; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
Hybridoma:
P3-NS1/1-Ag4-1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG3 | 10F588 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 250ug | | -20°C | Blue Ice | Bovine | Mouse |
|
| Concentration: |
| ~0.1mg/ml |
| Immunogen: |
| Theileria parva infected bovine lymphoblastoid cell line. |
| Purity: |
| Purified by Protein G affinity chromatography. |
| Form |
| Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 1% BSA. |
| Specificity: |
| Recognizes the bovine WC14 cell surface antigen. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Naessens, J. et al. (1985) De novo expression of T cell markers on Theileria parva- transformed lymphoblasts in cattle. J. Immunol. 135: 4183-4188. 2. Muiya, P. et al. (1993) Expression of antigens on haemopoietic progenitor cells in bovine bone marrow. Vet Immunol Immunopathol. 39:237-48. 3. Naessens, J. et al. (1996) Biochemical analysis of preliminary clusters in the non-lineage panel. Vet. Immunol. Immunopathol. 52: 347-356. 4. Howard, C.J. et al. (1996) Ruminant cluster WC14. Vet. Immunol. Immunopathol. 52: 261-262.
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