Technical Data
W0482-14
WC14
Description:
WC14 is expressed on monocytes and granulocytes, and a subset of activated T cells.

Applications:
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat-1:5; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
P3-NS1/1-Ag4-1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG310F588Affinity Purified
SizeStorageShippingSourceHost
250ug4C Do Not FreezeBlue IceBovineMouse
Concentration:
~0.1mg/ml
Immunogen:
Theileria parva infected bovine lymphoblastoid cell line.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 1% BSA.
Specificity:
Recognizes the bovine WC14 cell surface antigen.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Naessens, J. et al. (1985) De novo expression of T cell markers on Theileria parva- transformed lymphoblasts in cattle. J. Immunol. 135: 4183-4188. 2. Muiya, P. et al. (1993) Expression of antigens on haemopoietic progenitor cells in bovine bone marrow. Vet Immunol Immunopathol. 39:237-48. 3. Naessens, J. et al. (1996) Biochemical analysis of preliminary clusters in the non-lineage panel. Vet. Immunol. Immunopathol. 52: 347-356. 4. Howard, C.J. et al. (1996) Ruminant cluster WC14. Vet. Immunol. Immunopathol. 52: 261-262.