Technical Data
WD repeat domain 5 (WDR5, BIG3, BIG-3, BMP2-induced 3-kb gene protein, SWD3, WD repeat-containing protein 5)
WDR5 (WD repeat-containing protein 5; also BMP2-induced 3 kb gene protein/BIG3) is a
nuclear, 36-40kD monomeric member of the WD family of repeat proteins. It is known to be
expressed in osteoblasts, osteocytes and chrondrocytes, among other cells, and appears to
serve as a structural organizer for the histone methyltransferase MLL1 complex. This complex
methylates histone H3, and within this complex, WDR5 binds to mono- or dimethylated histone
H3 and serves as an anchor for complex subunits RbBP5, Ash2L and MLL1. Human WDR5 is
334 amino acids in length. It contains seven WD domains (aa43-333) that likely participate in protein-protein interactions, plus a phosphorylation site at Ser267. There are three isoform variants for WDR5. Human and mouse WDR5 are absolutely identical in aa sequence.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.02% sodium azide, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Not determined
Recombinant human WDR5, aa1-120.
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.02% sodium azide.
Recognizes endogenous human WDR5 at ~40kD. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.