Technical Data
W1006-12
WD repeat domain 5 (WDR5, BIG3, BIG-3, BMP2-induced 3-kb gene protein, SWD3, WD repeat-containing protein 5)
Description:
WDR5 (WD repeat-containing protein 5; also BMP2-induced 3 kb gene protein/BIG3) is a
nuclear, 36-40kD monomeric member of the WD family of repeat proteins. It is known to be
expressed in osteoblasts, osteocytes and chrondrocytes, among other cells, and appears to
serve as a structural organizer for the histone methyltransferase MLL1 complex. This complex
methylates histone H3, and within this complex, WDR5 binds to mono- or dimethylated histone
H3 and serves as an anchor for complex subunits RbBP5, Ash2L and MLL1. Human WDR5 is
334 amino acids in length. It contains seven WD domains (aa43-333) that likely participate in protein-protein interactions, plus a phosphorylation site at Ser267. There are three isoform variants for WDR5. Human and mouse WDR5 are absolutely identical in aa sequence.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.02% sodium azide, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
Not determined
Immunogen:
Recombinant human WDR5, aa1-120.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.02% sodium azide.
Specificity:
Recognizes endogenous human WDR5 at ~40kD. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.