Technical Data
W1018-40A
West Nile Virus, Core (WNV Core, Capsid Protein C)
Description:
West Nile Virus (WNV) is a member of the Flaviviridae, a plus-stranded virus family that includes St. Louis encephalitis virus, yellow fever virus, and Dengue virus. WNV was initially isolated in 1937 in the West Nile region of Uganda and has become prevalent in Africa, Asia and Europe. It has rapidly spread across the United States with cases being observed in every continental state. Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope embedded with envelope and matrix proteins which play a major role for WNV entry into target cells. The viral core protein is thought to contribute to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway as delivery of core gene delivery into the striatum of mouse brain and skeletal muscle resulted in cell death and inflammation.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1ug/ml will detect 10ng of free peptide.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ug4C Do Not FreezeBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 15aa near the amino-terminus of the WNV Core protein (NP_776011).
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes the core of West Nile Virus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gould LH and Fikrig E. West Nile virus: a growing concern? J. Clin. Invest. 2004; 113:1102-7. 2. Chu JJ, Rajamanonmani R, Li J, et al. Inhibition of West Nile virus entry by using a recombinant domain III from the envelope glycoprotein. J. Gen. Virol. 2005; 86:405-12. 3. Chu JJ and Ng ML. Interaction of West Nile virus with alpha v beta 3 integrin mediates virus entry into cells. J. Biol. Chem. 2004; 279:54533-41. 4. Yang JS, Ramanathan MP, Muthumani K, et al. Induction of inflammation by West Nile Virus capsid through the caspase-9 apoptotic pathway. Emerg. Infect. Dis. 2002; 8:1379-84.