Technical Data
W1018-40B
West Nile Virus, Core (WNV Core, Capsid Protein C)
Description:
West Nile Virus (WNV) is a member of the Flaviviridae, a plus-stranded virus family that includes St. Louis encephalitis virus, yellow fever virus, and Dengue virus. WNV was initially isolated in 1937 in the West Nile region of Uganda and has become prevalent in Africa, Asia, and Europe. It has rapidly spread across the United States with cases being observed in every continental state. Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope embedded with envelope and matrix proteins which play a major role for WNV entry into target cells. The viral core protein is thought to contribute to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway as delivery of core gene delivery into the striatum of mouse brain and skeletal muscle resulted in cell death and inflammation.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1ug/ml detects 10ng of free peptide
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 15aa near the C-terminal of the West Nile Virus core protein. (Genbank Accession #: NP_776011)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes West Nile Virus Core Protein, C-terminal.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gould, LH., et al., (2004), "West Nile virus: a growing concern?", J. Clin Invest., 113: 1102-1107. 2. Chu, J.J., et al., (2005), "Inhibition of West Nile virus entry by using a recombinant domain III from the envelope glycoprotein", J. Gen. Virol., 86: 405-412. 3. Chu, J.J., et al., (2004), "Interaction of West Nile virus with alpha v beta 3 integrin mediates virus entry into cells", J. Biol. Chem., 279: 54533-54541. 4. Yang, J.S., et al., (2002), "Induction of inflammation of West Nile Virus capsid through the caspase-9 apoptotic pathway", Emerg. Infect. Dis., 8: 1379-1384.