Technical Data
W1018-40D
West Nile Virus, Core, CT (WNV Core, Capsid Protein C)
Description:
WNV belongs to the Flaviviridae family which includes closely viruses such as Dengue fever, Yellow fever and Tick borne encephalitis. West Nile Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope containing envelope and matrix proteins. Research indicates that the viral core protein contributes to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway. West Nile Virus is believed to have originated in the West Nile region of Africa and has now spread to Europe, Asia and America where it has become a significant disease affecting human and domestic animal health.

Applications:
Suitable for use in ELISA, Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1ug/ml
Immunofluorescence: 20ug/ml
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
A peptide corresponding to a 15aa sequence from near the C-terminus of West Nile Virus core protein.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes the 14kD core protein of West Nile Virus (WNV).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gould, L.H., et al (2004) West Nile virus: a growing concern. J.Clin. Invest. 113:1102-1107 2. Chu, J.J., et al (2005) Inhibition of West Nile virus entry by using a recombinant domain III from the envelope glycoprotein. J. Gen. Virol. 86:405-12 3. Chu, J.J., et al (2004) Interaction of West Nile virus with a v ß 3 integrin mediates virus entry into cells. J. Biol. Chem. 2004; 279:54533-41. 4. Yang, J.S., et al (2002) Induction of inflammation by West Nile Virus capsid through the caspase-9 apoptotic pathway. Emerg. Infect. Dis. 8:1379-84.