		Technical Data
W1018-42C	West Nile Virus

Description:
West Nile (WN), the most widespread among flaviviruses, was first isolated from the serum of a febrile woman in 1937 in the West Nile district of Uganda. West Nile virus was first detected in North America in 1999 and has subsequently spread throughout the United States and Canada and into Mexico and the Caribbean. In Africa, southern Europe, western Asia, and the United States, WNV has been isolated from mosquitoes of more than 40 species. In the United States, Canada, and Israel, WNV is responsible for significant avian mortality.

Applications:
Suitable for use in ELISA and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:10-1:50
ELISA: 1:20-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	12K243	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice		Mouse

Concentration:

~0.1mg/ml

Immunogen:

Recombinant antigen to the West Nile Virus envelope

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

Specificity:

Recognizes West Nile Virus. Crossreacts with SLE, JEV and DEN. Does not crossreact with YFV, TBE, EEE, WEE, POW, LAC, or VEE.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.