Technical Data
W1018-50A
West Nile Virus, Envelope Protein E (West Nile Virus E Protein, WNV Envelope Glycoprotein E, WNVE)
Description:
West Nile (WN), the most widespread among flaviviruses, was first isolated from the serum of a febrile woman in 1937 in the West Nile district ofuganda. West Nile virus was first detected in North America in 1999 and has subsequently spread throughout the United States and Canada and into Mexico and the Caribbean. In Africa, southern Europe, western Asia, and the United States, WNV has been isolated from mosquitoes of more than 40 species. In the United States, Canada, and Israel, WNV is responsible for significant avian mortality.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
West Nile virus infected cell line

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to 73-86 (CPTMGEAHNEKRAD) of West Nile virus envelope glycoprotein E. (NC_001563).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes West Nile virus envelope glycoprotein E WNV E.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Smithburn JS, Hughes TP, Burke AW, Paul JH. A neurotropic virus isolated from the blood of a native of Uganda. Am. J. Trop. Med. Hyg. 20: 471492 (1970). 2. Hayes, C.G. West Nile fever. In The arboviruses: epidemiology and ecology. T.P. Monath, editor. CRC Press. Boca Raton, Florida, USA. 5988 (1989). 3. Yamshchikov,V.F., Wengler,G., Perelygin,A.A., Brinton,M.A. and Compans,R.W. An infectious clone of the West Nile flavivirus. Virology 281 (2), 294-304 (2001). 4. Laboratory-acquired West Nile virus infections United States, 2002. Morb. Mortal. Wkly Rep. 51:11331135 (2002). 5. L. Hannah Gould and Erol Fikrig. West Nile virus: a growing concern? J Clin Invest. 113(8): 11021107 (2004).