Technical Data
West Nile Virus, Envelope (CT) (West Nile Virus E Protein, WNV Envelope Protein, Envelope Protein, Genome Polyprotein, Major Envelope Protein E, WNVgp1)
West Nile Virus (WNV) is a member of the Flaviviridae, a plus-stranded virus family that includes St. Louis encephalitis virus, yellow fever virus, and Dengue virus. WNV was initially isolated in 1937 in the West Nile region of Uganda and has become prevalent in Africa, Asia, and Europe. It has rapidly spread across the United States with cases being observed in every continental state. Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope embedded with envelope and matrix proteins. While the viral core protein is thought to contribute to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway, the highly glycosylated envelope protein plays a major role for WNV entry into target cells as this entry can be inhibited by using a recombinant domain III from the envelope glycoprotein. The WNV receptor has recently been identified as alpha v beta 3 integrin.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceRabbit
Synthetic peptide corresponding to 14aa at the C-terminus of the West Nile Virus Envelope protein.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.02% sodium azide.
Recognizes West Nile Virus Envelope Protein, C-terminal.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gould, LH., et al., (2004), "West Nile virus: a growing concern?", J. Clin Invest., 113: 1102-1107. 2. Yang, J.S., et al., (2002), "Induction of inflammation by West Nile Virus capsid through the caspase-9 apoptotic pathway", Emerg. Infect. Dis., 8: 1379-1384. 3. Chu, J.J., et al., (2005), "Inhibition of West Nile virus entry by using a recombinant domain III from the envelope glycoprotein", J. Gen. Virol., 86: 405-412. 4. Chu, J.J., et al., (2004), "Interaction of West Nile virus with alpha v beta 3 integrin mediates virus entry into cells", J. Biol. Chem., 279: 54533-54541.