Technical Data
W1018-50K
West Nile Virus
Description:
West Nile Virus (WNV) is a member of the Flaviviridae, a plus-stranded virus family that includes St. Louis encephalitis virus, yellow fever virus, and Dengue virus. WNV was initially isolated in 1937 in the West Nile region of Uganda and has become prevalent in Africa, Asia, and Europe. It has rapidly spread across the United States with cases being observed in every continental state (reviewed in. Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope embedded with envelope and matrix proteins which play a major role for WNV entry into target cells. The viral core protein is thought to contribute to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway as delivery of core gene delivery into the striatum of mouse brain and skeletal muscle resulted in cell death and inflammation.

Applications:
Suitable for use in Immunofluorescence and ELISA. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:10-1:50
ELISA: 1:20-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG110D33Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~0.1mg/ml
Immunogen:
Truncated recombinant corresponding to the envelope antigen of West Nile Virus.
Purity:
Purified by Protein A affinity chromatography (>90%).
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No Stabilizing proteins added.
Specificity:
Recognizes the envelope protein of West Nile Virus. Positive reaction with WNV and SLE flaviviruses. Negative reaction with JEV, YFV, DEN, TBE, EEE, WEE, POW, LAC, VEE.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.