Technical Data
West Nile Virus, NS3 Protease (WNV)
The trypsin-like protease, NS3, and its co-factor, NS2b, play an essential role in replication and are therefore potential targets for treatment and prevention of West Nile Virus disease.

Suitable for use in Direct ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~2ng/well.
Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit is ~5ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5-50 fold.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG18H154Affinity Purified
100ug-20CBlue IceMouse
~0.5mg/ml (after reconstitution)
Recombinant protein corresponding aa1-184 of NS3 (NP_776018) and aa49-96 of NS2b (NP_776017) of West Nile Virus NS3 Protease, expressed in E. coli.
Purified by Protein G affinity chromatography.
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Recognizes West Nile Virus NS3 Protease.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.