Technical Data
W1018-54
West Nile Virus, NS3 Protease (WNV)
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Technical Data |
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W1018-54 |
West Nile Virus, NS3 Protease (WNV) |
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Description: The trypsin-like protease, NS3, and its co-factor, NS2b, play an essential role in replication and are therefore potential targets for treatment and prevention of West Nile Virus disease. Hybridoma: Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E.coli-derived, recombinant West Nile Virus NS3 Protease expressed as a fusion with the cofactor NS2b, (rwnvNS3, aa 1-184 of NS3, Accession # NP_776018, and aa 49-96 of NS2b, Accession # NP_776017). Applications: Western Blot, Direct ELISA Recommended Dilution: Western Blot: This antibody can be used at 1-2ug/mL with the appropriate secondary reagents to detect west nile virus NS3 protease. Using a colorimetric detection system, the detection limit for rwnvNS3 protease is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Direct ELISA: This antibody can be used at 0.5-1.0ug/mL with the appropriate secondary reagents to detect west nile virus NS3 protease. The detection limit for rwnvNS3 is approximately 2 ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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