Technical Data
W1018-54
West Nile Virus, NS3 Protease (WNV)
Description:
The trypsin-like protease, NS3, and its co-factor, NS2b, play an essential role in replication and are therefore potential targets for treatment and prevention of West Nile Virus disease.

Applications:
Suitable for use in Direct ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~2ng/well.
Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit is ~5ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5-50 fold.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18H154Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding aa1-184 of NS3 (NP_776018) and aa49-96 of NS2b (NP_776017) of West Nile Virus NS3 Protease, expressed in E.coli.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity:
Recognizes West Nile Virus NS3 Protease.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.