Technical Data
Media
Wilkins Chalgren Agar (Powder)
W1050
Microbiological Grade
Storage RT    Shipping RT

Highly nutritive medium for cultivation and isolation of aerobic and anaerobic bacteria.

Appearance:
Light beige homogenous, free fowing powder

Solubility:
Yellow-beige, trace to slight hazy

pH (25C):
7.1 0.2

Formulation (g/L):
Enzymatic Digest of Casein 10g
Enzymatic Digest of Gelatin 10g
Yeast Extract 5g
Sodium Chloride 5g
Dextrose 1g L-Arginine 1g
Sodium Pyruvate 1g
Hemin 0.005g
Vitamin K 0.0005g
Agar 15g

Total 47.0055

Directions per Liter:
Dissolve 47.0055g in 1L of ddH2O. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Dispense into appropriate containers, loosen caps and autoclave (15psi) for 15 minutes at 121C.

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.



Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Wilkins, T. D., and Chalgren, S., Antimicrob. Agents. Chemother. 10: 926 (1976). 2. Balows, A., W.J. Hausler, Jr., K.L. Herrmann, H.D. Isenberg, and H.J. Shadmony (eds.). 1991. Manual of clinical microbiology, 5th ed. American Society for Microbiology, Washington, D.C. 3. Smith, L.D.S., 1975. The pathogenic anaerobic bacteria, 2nd ed. Charles C. Thomas, Springfield, Ill. 4. Isenberg, H.D., (ed.). 1992. Clinical microbiology procedures handbook. American Society for Microbiology, Washington, D.C. 5. Murray, P.R., E.J. Baron, M.A. Pfaller, F.C. Tenover, and R.H. Yolken (eds.). 1995. Manual of clinical microbiology, 6th ed. American Society for Microbiology, Washington, D.C. 6. Baron, E.J., L.R. Peterson, and S.M. Finegold. 1994. Bailey & Scotts diagnostic microbiology, 9th ed. Mosby-Year Book, Inc., St. Louis, MO. 7. www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalmanualBAM/default.htm. 8. Vanderzant, C., and D.F. Splittstoesser (eds.). 1992. Compendium of methods for the microbiological examination of food, 3rd ed. American Public Health Association, Washington, D.C.