Technical Data
Wilkins-Chalgren Broth (Powder)
Microbiological Grade
Storage RT/4C    Shipping RT

Highly nutritive medium for cultivation and isolation of aerobic and anaerobic bacteria.

Light beige homogenous, free fowing powder

Amber, clear

7.1 0.2

Formulation (g/L):
Enzymatic Digest of Casein 10.0
Enzymatic Digest of Gelatin 10.0
Yeast Extract 5.0
Sodium Chloride 5.0
Dextrose 1.0
L-Arginine 1.0
Sodium Pyruvate 1.0
Hemin 0.0050
Vitamin K 0.0005
Total 33.0

Directions per Liter:
Dissolve 33g in 1L of ddH2O. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Dispense into appropriate containers, loosen caps and autoclave ((15psi) for 15 minutes at 121C.

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Wilkins, T. D., and S. Chalgren. 1976. Antimicrob. Agents. Chemother. 10:926. 2. Balows, A., W. J. Hausler, Jr., K. L. Herrmann, H. D. Isenberg, and H. J. Shadmony (eds.). 1991. Manual of clinical microbiology, 5th ed. American Society for Microbiology, Washington, D.C. 3. Smith, L. D. S. 1975. The pathogenic anaerobic bacteria, 2nd ed. Charles C. Thomas, Springfield, Ill. 4. Isenberg, H. D. (ed.). 1992. Clinical microbiology procedures handbook. American Society for Microbiology, Washington, D.C. 5. Murray, P. R., E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (eds.). 1995. Manual of clinical microbiology, 6th ed. American Society for Microbiology, Washington, D.C. 6. Baron, E. J., L. R. Peterson, and S. M. Finegold. 1994. Bailey & Scotts diagnostic microbiology, 9th ed. Mosby-Year Book, Inc., St. Louis, MO. 7. Association of Official Analytical Chemists. 1995. Bacteriological analytical manual, 8th ed. AOAC International, Gaithersburg, MD. 8. Vanderzant, C., and D. F. Splittstoesser (eds.). 1992. Compendium of methods for the microbiological examination of food, 3rd ed. American Public Health Association, Washington, D.C.