Technical Data
W1450-50B
WISP2, ID (WNT1-inducible-signaling Pathway Protein 2, WISP-2, Connective Tissue Growth Factor-like Protein, CTGF-L, Connective Tissue Growth Factor-related Protein 58, CCN Family Member 5, CCN5, CT58, CTGFL, UNQ228/PRO261)
Description:
Wisp2 a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. Wisp2 lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the center region of human WISP2 (KLH).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human WISP2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003). 2.Banerjee, S., et al., Neoplasia 5(1):63-73 (2003). 3.Kumar, S., et al., J. Biol. Chem. 274(24):17123-17131 (1999). 4.Pennica, D., et al., Proc. Natl. Acad. Sci. USA 95(25):14717-14722 (1998). 5.Saxena, N., et al., Mol. Cell. Biochem. 228(1-2), 99-104 (2001).