Technical Data
W1450-53
WISP3, ID (WNT1-inducible-signaling Pathway Protein 3, WISP-3, CCN Family Member 6, CCN6, UNQ462/PRO790/PRO956)
Description:
WISP3 is a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. WISP3 is overexpressed in colon tumors. It may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Mutations of the WISP3 gene are associated with progressive pseudorheumatoid dysplasia, an autosomal recessive skeletal disorder, indicating that the gene is essential for normal postnatal skeletal growth and cartilage homeostasis.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:200
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the center region of human WISP3 (KLH).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human WISP3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003). 2.Tanaka, S., et al., Gastroenterology 123(1):392-393 (2002). 3.Kleer, C.G., et al., Oncogene 21(20):3172-3180 (2002). 4.Hurvitz, J.R., et al., Nat. Genet. 23(1):94-98 (1999). 5.Pennica, D., et al., Proc. Natl. Acad. Sci. USA 95(25):14717-14722 (1998).