Technical Data
Wnt1 (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)
Wnts have diverse roles in governing cell fate, proliferation, migration, porarity and death. Wnt signaling controls development and is important for embryogenesis and carcinogenesis. Wnts are transduced through at least three distinct intracellular signaling pathways including the canonical ‘Wnt/b-catenin’, ‘Wnt/Ca2+’ and ‘Wnt’ polarity (also called the ‘planar polarity’ pathway) pathways. Distinct sets of Wnt and Frizzled ligand-receptor pairs activate each of
these pathways and lead to unique cellular responses. The Wnt/b-catenin pathway primarily regulates cell fate determination during development, whereas the main function of the Wnt polarity pathway is the regulation of cytoskeletal organization. The molecular weight of Wnt1 is 41kD. Wnt1 has a role in melanocyte expansion and differentiation during mouse development. Mouse proto-oncogene Wnt1 is activated by proviral insertion of mouse mammary tumor virus (MMTV). Transgenic expression of mouse Wnt1 using MMTV LTR enhancer causes mammary adenocarcinomas in approximately 50% of female transgenic mice by 12 months of age. Wnt1 induces mouse mammary carcinogenesis even in estrogen receptor alpha knockout mice. The interaction between b-catenin and EGFR/c-neu heterodimers in Wnt1-mediated breast tumor genesis is indispensable, thus it may be a fundamental signaling event in human metastatic progression. Wnt1 is expressed in human gastric, pancreatic and breast cancers.

Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml
Immunoprecipitation: 7ug/reaction
Optimal dilutions to be determined by the researcher

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ug-20°CBlue IceHumanRabbit
Synthetic peptide derived from the C-terminal region of the human Wnt1 protein.
Purified by epitope affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide
Reacts with the human and mouse Wnt1 proteins. On Western blots, it identifies a single band at ~41kD. A band of unknown origin at ~80-90kD is sometimes observed; this may represent a dimerized form of Wnt1. Reactivity has been confirmed with Wnt1-transfected HEK293 cells, GIST882 and SW480 cell lysates, and mouse MS-1 cell lysates.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.